赵仲华   博士
	教授   博士生导师
	Email：zhzhao@sxu.edu.cn

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招生专业

博士研究生：遗传学

学术性硕士研究生：遗传学

专业性硕士研究生：生物技术与工程

主要研究方向

本课题组主要关注的科学问题是Hedgehog（Hh）信号转导通路的分子机制与Hh通路相关疾病病理机制。Hh通路在大多数动物中高度保守。其在动物胚胎发育、形态建成和组织器官发生，以及成体物质能量代谢稳定中发挥重要的作用。对其机制的研究有助于我们对于胚胎发育、器官形成的理解，同时可以揭示Hh通路相关疾病的致病机制，发现新的干预靶点，具有重要的科学意义和潜在应用价值。

同时我们基于斑马鱼模型开展神经系统遗传性疾病病理机制的研究，以及以斑马鱼为模型的新型药物筛选、开发和评价的研究。

目前课题组主要开展的研究方向如下：

1.      脊椎动物Hh通路SMO下游的信号转导分子机制以及相关疾病病理机制的研究。

主要针对Hh通路激活后纤毛对其信号转导调控的机制开展研究，包括纤毛中PKA的活性、KIF7的功能等。同时以KIF7突变引起的Acrocallosal综合征为切入点，通过KIF7翻译后修饰以及互作组学的研究揭示KIF7突变导致神经发育缺陷的病理机制。

2.      遗传性神经发育缺陷疾病病理机制研究

主要开展以斑马鱼为模型，探究wdr45缺失导致神经退行性疾病病理机制的研究。

3.      新型药物筛选和评价的研究

主要以斑马鱼为模型，建立多种疾病模型，对各类型药物开展评价。

承担项目情况

1.      山西省中央引导地方自由探索类项目，初级纤毛靶向功能蛋白递送方法的建立及在Hh通路相关疾病治疗中的研究，在研，主持。

2.      山西省省筹资金资助留学归国人员科技项目, 2024-011, 清肺排毒汤治疗新型冠状病毒感染作用机理的研究,在研，主持。

3.      国家自然科学基金，地区联合基金，基于人工智能影像的垂体腺瘤多组学融合精准诊疗预测模型研发，在研，主要参与人。

4.      国家自然科学基金, 面上项目, GRK2-GPR161协同调控Hh通路信号传递分子机制的研究, 结题，主持；

5.      山西省科技厅，社会发展重点研发计划，Hh通路相关出生缺陷潜在遗传变异快速功能检测方法的建立与应用研究，结题，主持；

6.      山西省人社厅，山西省归国留学人员科技活动择优项目，重点项目，GRK2磷酸化修饰Gpr161位点的鉴定及生物学功能的研究，结题，主持；

7.      山西省委组织部，山西省百人计划，青年项目，结题，主持；

8.      国家自然科学基金, 青年项目, Gα蛋白在Hedgehog信号转导通路中的功能，结题，主持；

发表论文情况

1.    Zhang H, Huang Z, Lv L, Xin Y, Wang Q, Li F, Dong L, Wu C, Ingham PW, Zhao Z. A transgenic zebrafish for in vivo visualization of cilia. Open Biol. 2022 Aug;12(8):220104. doi: 10.1098/rsob.220104. Epub 2022 Aug 10. PMID: 35946311; PMCID: PMC9364149.

2.    Wang Y, Bo J, Zhao Z, Han Y, Zhang Q, Liu L. Depletion of Igfbp7 alleviates zebrafish NAFLD progression through inhibiting hepatic ferroptosis. Life Sci. 2023 Nov 1;332:122086. doi: 10.1016/j.lfs.2023.122086. Epub 2023 Sep 14. PMID: 37714372.

3.    Cui J, Wen D, Wang L, Meng C, Wang Y, Zhao Z, Wu C. CRISPR/Cas9-induced asap1a and asap1b co-knockout mutant zebrafish displayed abnormal embryonic development and impaired neutrophil migration. Gene Expr Patterns. 2023 Sep;49:119331. doi: 10.1016/j.gep.2023.119331. Epub 2023 Jun 29. PMID: 37390886.

4.    Dong L, Fan X, Li Q, Li J, Chen G, Yang D, Li Y, Zhao L, Yin H, Wang X, Liu X, Zhao Z, Lin S. Anemone chinensis Bunge aqueous enema alleviates dextran sulfate sodium-induced colitis via inhibition of inflammation and regulation of the colonic mucosal microbiota. J Ethnopharmacol. 2022 Apr 24;288:114916. doi: 10.1016/j.jep.2021.114916. Epub 2022 Jan 5. PMID: 34998930.

5.    Xiong Q, Sun H, Wang Y, Xu Q, Zhang Y, Xu M, Zhao Z, Li P, Wu C. Lipid droplet accumulation in Wdr45-deficient cells caused by impairment of chaperone-mediated autophagic degradation of Fasn. Lipids Health Dis. 2024 Mar 28;23(1):91. doi: 10.1186/s12944-024-02088-y. PMID: 38539242; PMCID: PMC10976834.

6.    Chen G, Wen D, Shen L, Feng Y, Xiong Q, Li P, Zhao Z. Cepharanthine Exerts Antioxidant and Anti-Inflammatory Effects in Lipopolysaccharide (LPS)-Induced Macrophages and DSS-Induced Colitis Mice. Molecules. 2023 Aug 15;28(16):6070. doi: 10.3390/molecules28166070. PMID: 37630322; PMCID: PMC10458559.

7.    Xiong Q, Sun H, Xing W, Li X, Chen G, Zhao Z, Wu C, Li P. WDR45 mutation dysregulates iron homeostasis by promoting the chaperone-mediated autophagic degradation of ferritin heavy chain in an ER stress/p38 dependent mechanism. Free Radic Biol Med. 2023 May 20;201:89-97. doi: 10.1016/j.freeradbiomed.2023.03.012. Epub 2023 Mar 20. PMID: 36940732.

8.    Cui J, Chen G, Zhao Z, Wang L, Wen D, Xing L, Wu C. ASAP1 regulates the uptake of Mycobacterium tuberculosis H37Ra in THP1-derived macrophages by remodeling actin cytoskeleton. Tuberculosis (Edinb). 2021 Jul;129:102090. doi: 10.1016/j.tube.2021.102090. Epub 2021 May 26. PMID: 34058694.

9.    Cui J, Chen G, Wen D, Wang Y, Zhao Z, Wu C. Asap1 Affects the Susceptibility of Zebrafish to Mycobacterium by Regulating Macrophage Migration. Front Cell Infect Microbiol. 2020 Oct 29;10:519503. doi: 10.3389/fcimb.2020.519503. PMID: 33194781; PMCID: PMC7658321.

10. Chang D, Zhao Z, Shi L, Liu W, Yang Y. Lysosome-targeted carbon dots for colorimetric and fluorescent dual mode detection of iron ion, in vitro and in vivo imaging. Talanta. 2021 Sep 1;232:122423. doi: 10.1016/j.talanta.2021.122423. Epub 2021 Apr 22. PMID: 34074409.

11. Wang M, Li P, Wang H, Dong L, Wu C, Zhao Z. Identification and spatiotemporal expression of gpr161 genes in zebrafish. Gene. 2020 Mar 10;730:144303. doi: 10.1016/j.gene.2019.144303. Epub 2019 Dec 26. PMID: 31884103.

12. Zhonghua Zhao, Raymond Teck Ho Lee, Ganesh Pusapati, Audrey Iyu, Rajat Rohatgi, and Philip Ingham. (2015) An essential Role for Grk2 in Hedgehog signalling downstream of Smoothened. EMBO Reports, doi: 10.15252/embr.201541532.

13. Raymond Tech Ho Lee*, Zhonghua Zhao* and Philip W. Ingham. (2015) Development at a glance: Hedgehog signaling. Development, 143(3): 367-372.

14. 3. Ashish K. Maurya*, Jin Ben*, Zhonghua Zhao*, Raymond Lee, Weixin Niah, Ashley Shu Mei Ng, Stone Elworthy, Fredericus JM van Eeden and Philip W. Ingham. (2013) Modulation of Kif7 sub-cellular distribution by Hedgehog activity mediates the regulation of Gli transcription factor activity in the zebrafish. Plos Genetics, 9(12): p e1003955

15. Andreas van Impel, Zhonghua Zhao, Dorien M. A. Hermkens, M. Guy Roukens, Johanna C. Fischer, Josi Peterson-Maduro, Henricus Duckers, Elke A. Ober, Philip W. Ingham, and Stefan Schulte-Merker. (2014) Divergence of zebrafish and mouse lymphatic cell fate specification pathways. Development,141:1228-1238.

16. Sigrid Nachtergaele, Daniel M. Whalen, Laurel K. Mydock, Zhonghua Zhao, Tomas Malinauskas, Kathiresan Krishnan, Philip W. Ingham, Douglas F. Covey and Christian Siebold. (2013) Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling. Elife, 2: p.e01340.

17. Xingang Wang*, Zhonghua Zhao*, Julius Muller, Audrey Iyu, Alexis Jiaying Khng, Ernesto Guccione, Yijun Ruan, and Philip W. Ingham. (2013) Targeted inactivation and identification of targets of the Gli2a transcription factor in the zebrafish. Biology open, 2 (11): 1203-1213.

18. Li Zhang, Yumin Hao, Yang Liu, Yueping Dong, Zexian Chen, Wenjuan Dong, Zhonghua Zhao, Qin Hu, Chuan Dong, Xiaojuan Gong. Nickel doping: Improving the optical properties of carbon dots and increasing the sensitivity for detecting pH and water,Journal of Alloys and Compounds, 2024, 976,173131,

19. Lan Zhao*, Jian Huang*, Zhonghua Zhao*, Qun Li, Yongbiao Xue. (2010) The Skp1-like protein SSK1 is required for cross-pollen compatibility in SRNase- based self-incompatibility. Plant Journal, 62(1):52-63.

20. Guang Chen, Bin Zhang, Zhonghua Zhao, Zhenghua Sui, Hui Zhang and Yongbiao Xue. (2010) A life or death decision' for pollen tubes in S RNasebased self-incompatibility. Journal of Experimental Botany, 61(7):2027-37.

21. Yijing Zhang, Zhonghua Zhao and Yongbiao Xue. (2009) Roles of proteolysis inplant self-incompatibility. Annual Review of Plant Biology, 60:21-42.